- HPA002926 - Atlas Antibodies KLF4 antibody

Submitted references Differentiation-Dependent KLF4 Expression Promotes Lytic Epstein-Barr Virus Infection in Epithelial Cells

Nawandar D, Wang A, Makielski K, Lee D, Ma S, Barlow E, Reusch J, Jiang R, Wille C, Greenspan D, Greenspan J, Mertz J, Hutt-Fletcher L, Johannsen E, Lambert P, Kenney S, Speck S
PLOS Pathogens 2015 October;11(10)

ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes.

Boxer LD, Barajas B, Tao S, Zhang J, Khavari PA
Genes & development 2014 Sep 15;28(18):2013-26

A study of embryonic stem cell-related proteins in human astrocytomas: Identification of Nanog as a predictor of survival

Elsir T, Edqvist P, Carlson J, Ribom D, Bergqvist M, Ekman S, Popova S, Alafuzoff I, Ponten F, Nistér M, Smits A
International Journal of Cancer 2014 March;134(5):1123-1131

Endothelial Kruppel-like factor 4 regulates angiogenesis and the Notch signaling pathway.

Hale AT, Tian H, Anih E, Recio FO 3rd, Shatat MA, Johnson T, Liao X, Ramirez-Bergeron DL, Proweller A, Ishikawa M, Hamik A
The Journal of biological chemistry 2014 Apr 25;289(17):12016-12028

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Western blot analysis in human cell lines PC-3 and U2OS using Anti-KLF4 antibody. Corresponding KLF4 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Orthogonal method: Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and KLF4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401356).

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human skin and pancreas tissues using HPA002926 antibody. Corresponding KLF4 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skin shows moderate to strong nuclear positivity in keratinocytes.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human colon shows moderate to strong nuclear positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Sample type: HUMAN

HPA002926

Antibody data