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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Flow cytometry [3]
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- Product number
- F53713 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD138 Antibody
- Antibody type
- Monoclonal
- Antigen
- A portion of amino acids 210-238 from the human protein was used as the immunogen for this CD138 antibody.
- Description
- Ascites
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 587CT7.3.6.5
- Vial size
- 100 µl
- Storage
- Aliquot the CD138 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- CD138 antibody western blot analysis in HepG2 lysate. Predicted molecular weight: 20-95 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of CD138 antibody with U266 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of CD138 antibody with T47D cells followed by secondary Ab (green). DAPI was used as a nuclear counterstain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of CD138 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS: CD138 antibody flow cytometric analysis of U266 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- CD138 antibody flow cytometric analysis of T47D cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- CD138 antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
