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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- MA5-27769 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFKBIL2 Monoclonal Antibody (GT648)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, HeLa, HepG2
- Antibody clone number
- GT648
- Concentration
- 1 mg/mL
Submitted references Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.
Jackson LM, Dhoonmoon A, Hale A, Dennis KA, Schleicher EM, Nicolae CM, Moldovan GL
Nucleic acids research 2021 Dec 16;49(22):12855-12869
Nucleic acids research 2021 Dec 16;49(22):12855-12869
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFKBIL2 in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate, D) HepG2 whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with NFKBIL2 monoclonal antibody (Product # MA5-27769) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NFKBIL2 Monoclonal Antibody (GT648) (Product # MA5-27769). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NFKBIL2 Monoclonal Antibody (GT648) (Product # MA5-27769) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
