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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-82733 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GFR alpha-3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LPTESRLMNS CLQARRKCQA DPTCSAAYHH LDSCTSSIST PLPSEEPSVP ADCLEAAQQL RNSSLIGCMC HRRMKNQVAC L
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GFR alpha-3 by a GFR alpha-3 polyclonal antibody (Product # PA5-82733). Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400574).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GFR alpha-3 in U-2 OS cells using a GFR alpha-3 polyclonal antibody (Product # PA5-82733). The analysis shows localization to plasma membrane & cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GFR alpha-3 in human testis using a GFR alpha-3 polyclonal antibody (Product # PA5-82733). The analysis shows strong cytoplasmic positivity in cells in seminiferous ducts.
