H00002875-M03

antibody from Abnova Corporation

Targeting: GPT ALT1, GPT1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002875-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002875-M03, RRID:AB_606315

Product name

GPT monoclonal antibody (M03), clone 4E2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant GPT.

Antigen sequence

MASSTGDRSQAVRHGLRAKVLTLDGMNPRVRRVEY
AVRGPIVQRALELEQELRQGVKKPFTEVIRANIGD
AQAMGQRPITFLRQVLALCVNPDLLSSPNFPDDAK
KRAERILQACGGHSLGAYSVSSGIQLIREDVARYI
ERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGE
GHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDE
ERAWALDVAELHRALGQARDHCRPRALCVINPGNP
TGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVY
AAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKG
YMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCP
PVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAE
LAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQL
PPRAVERAQELGLAPDMFFCLRLLEETGICVVPGS
GFGQREGTYHFRMTILPPLEKLRLLLEELSRFHAK
FTLEYS


Isotype

IgG

Antibody clone number

4E2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GPT expression in transfected 293T cell line by GPT monoclonal antibody (M03), clone 4E2.Lane 1: GPT transfected lysate(55 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FLJ23834 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol