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Learn11177-1-AP
antibody from Invitrogen Antibodies
Targeting: OGFR
7-60
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 11177-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OGFR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LERSQGDEA GGHGEDRPEP LSPKESKKRK LELSRREQPP TEPGPQSASE VEKIALNLEG CALSQGSLRT GTQEVGGQDP GEAVQPCRQP LGARVADKVR KRRKVDEGAG DSAAVASGGA QTLALAGSPA PSGHPKAGHS ENGVEEDTEG RTGPKEGTPG SPSETPGPSP AGPAGDEPAE SPSETPGPRP AGPAGDEPAE SPSETPGPSP AGPTRDEPAE SPSETPGPRP AGPAGDEPAE SPSETPGPRP AGPAGDEPAE SPSETPGPSP AGPTRDEPAK AGEAAELQDA EVESSAKSGK P (358-658 aa encoded by BC014137)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COLO 320 cells using 11177-1-AP (OGFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of L02 cells using 11177-1-AP (OGFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 11177-1-AP (OGFR antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HEK-293 cells were stained with 0.2ug OGFR antibody (11177-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-OGFR (IP:11177-1-AP, 5ug; Detection:11177-1-AP 1:500) with COLO 320 cells lysate 1200ug.
