TA502866

antibody from OriGene

Targeting: PPM1B PP2CB, PP2CBETA, PPC2BETAX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502866

Provider

OriGene

Proper citation

OriGene Cat#TA502866, RRID:AB_2622386

Product name

PPM1B mouse monoclonal antibody, clone OTI3C9 (formerly 3C9)

Antibody type

Monoclonal

Description

PPM1B mouse monoclonal antibody, clone OTI3C9 (formerly 3C9)

Host

Mouse

Conjugate

Unconjugated

Epitope

PPM1B

Isotype

IgG

Antibody clone number

OTI3C9

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PPM1B mouse monoclonal antibody (TA502866) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPM1B(RC212918 ).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-PPM1B antibody(TA502866),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC212918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPM1B antibody(TA502866), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-PPM1B antibody(TA502866),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC