Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA502865 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502865, RRID:AB_11124020
- Product name
- PPM1B mouse monoclonal antibody, clone OTI3F2 (formerly 3F2)
- Antibody type
- Monoclonal
- Description
- PPM1B mouse monoclonal antibody, clone OTI3F2 (formerly 3F2)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PPM1B
- Isotype
- IgG
- Antibody clone number
- OTI3F2
- Vial size
- 100 µl
- Concentration
- 0.7 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1B monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-PPM1B antibody(TA502865),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-PPM1B antibody(TA502865),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC212918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPM1B antibody(TA502865), and then analyzed by flow cytometry.
- Validation comment
- FC
