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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX81956 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81956, RRID:AB_11173709
- Product name
- EGR1 antibody, N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references REM sleep deprivation-induced circadian clock gene abnormalities participate in hippocampal-dependent memory impairment by enhancing inflammation in rats undergoing sevoflurane inhalation.
Hou J, Shen Q, Wan X, Zhao B, Wu Y, Xia Z
Behavioural brain research 2019 May 17;364:167-176
Behavioural brain research 2019 May 17;364:167-176
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of EGR1 Antibody (N-term) (GTX81956) in mouse NIH-3T3 tissue lysates (35μg/lane). EGR1 (arrow) was detected using the purified Pab.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EGR1 Antibody (N-term) (GTX81956) western blot analysis in MDA-MB231 cell line lysates (35μg/lane).This demonstrates the EGR1 antibody detected the EGR1 protein (arrow).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of EGR1 Antibody (N-term)(GTX81956) with hela cell followed by Alexa Fluor 488-conjμgated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EGR1 Antibody (N-term) (GTX81956) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
