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LearnF48309
antibody from NSJ Bioreagents
Targeting: EGR1
AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F48309 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EGR1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 9-37 from the human protein was used as the immunogen for this EGR1 antibody.
- Description
- Purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the EGR1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- EGR1 antibody western blot analysis in MDA-MB231 lysate. Predicted molecular weight ~58 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of EGR1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- EGR1 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
