PAB29383

antibody from Abnova Corporation

Targeting: CEBPE CRP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29383

Provider

Abnova Corporation

Product name

CEBPE polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human CEBPE.

Antigen sequence

SHGTYYECEPRGGQQPLEFSGGRAGPGELGDMCEH
EASIDLSAYIESGEEQLLSDLFAVKPAPEARGLKG
PGTPAFPHYLPPDPRPFAYPPHTFGPDRKALGPGI
YSSPGSYDPRAVAVKEEPR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with CEBPE polyclonal antibody (Cat # PAB29383) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with CEBPE polyclonal antibody (Cat # PAB29383) at 1-4 ug/mL concentration shows positivity in nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow with CEBPE polyclonal antibody (Cat # PAB29383) shows strong nuclear positivity in bone marrow poietic cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)