MAB513

antibody from Novus Biologicals

Targeting: MMP3 STMY, STMY1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB513

Provider

Novus Biologicals

Product name

Mouse Monoclonal MMP-3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects both the pro and active forms of human MMP-3 in direct ELISAs and Western blots. In direct ELISAs, 50-100% cross-reactivity with recombinant mouse MMP-3 is observed, 10% cross-reactivity with recombinant human (rh) MMP-10 is observed and no cross-reactivity with rhMMP-1, -2, -7, -8, -9, -12 or -13 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.

Villeneuve J, Block A, Le Bousse-Kerdilès MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
Experimental hematology 2009 Jul;37(7):849-56

---

IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.

Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65

---

IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.

Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65

---

Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.

Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut 2006 Dec;55(12):1774-80

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human MMP-3 by Western Blot. Western blot shows Recombinant Human MMP-3 Westrern Blot Standard Protein (2 μL, Catalog # WBC015). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-3 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

MMP-3 in Human Prostate and Human Prostate Cancer Tissue. MMP-3 was detected in immersion fixed paraffin-embedded sections of normal human prostate and human prostate cancer tissue using Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells (right panel). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of MMP-3 in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.