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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Flow cytometry [1]
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- Product number
- NBP2-61677 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against human CHRNA5 (AA: 23-254) recombinant protein. (Expected MW is 52.5 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mouse mAb against membrane protein lysate of C6 (1), membrane protein lysate of SK-N-SH (2), membrane protein lysate of C6 (3), and C6 (4) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against HEK293 (1) and CHRNA5 (AA: 23-254)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis of SK-N-SH cells using CHRNA5 mouse mAb (green) and negative control (red).
