600-401-936

antibody from Rockland Immunochemicals, Inc.

Targeting: CRTC1 FLJ14027, KIAA0616, MECT1, TORC1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-936

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-936, RRID:AB_2083834

Product name

Anti-MECT1 (RABBIT) Antibody - 600-401-936

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Dependency of human and murine LKB1-inactivated lung cancer on aberrant CRTC-CREB activation.

Zhou X, Li JW, Chen Z, Ni W, Li X, Yang R, Shen H, Liu J, DeMayo FJ, Lu J, Kaye FJ, Wu L
eLife 2021 Jun 18;10

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Altered LKB1/CREB-regulated transcription co-activator (CRTC) signaling axis promotes esophageal cancer cell migration and invasion.

Gu Y, Lin S, Li JL, Nakagawa H, Chen Z, Jin B, Tian L, Ucar DA, Shen H, Lu J, Hochwald SN, Kaye FJ, Wu L
Oncogene 2012 Jan 26;31(4):469-79

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-MECT2 antibody shows detection of MECT2 (lower arrowhead) and MECT2:MAML2 fusion protein (top arrowhead) in cell lysates. Lane 1 contains lysate from cells expressing MECT2 only. Lane 2 contains lysate from cells transfected with fusion protein. Lane 3 contains lysate from cells strongly expressing MECT2 and weakly expressing the fusion protein. Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Frederick Kaye, CCR-NCI, Bethesda, MD.

Validation comment

Western Blot