H00005094-M07

antibody from Novus Biologicals

Targeting: PCBP2 hnRNP-E2, HNRNPE2, HNRPE2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00005094-M07

Provider

Novus Biologicals

Proper citation

Novus Cat#H00005094-M07, RRID:AB_546797

Product name

Mouse Monoclonal PCBP2 Antibody

Antibody type

Monoclonal

Description

IgG purified. PCBP2 - poly(rC) binding protein 2

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Flavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses.

Roth H, Magg V, Uch F, Mutz P, Klein P, Haneke K, Lohmann V, Bartenschlager R, Fackler OT, Locker N, Stoecklin G, Ruggieri A
mBio 2017 Jan 10;8(1)

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Poly(rC)-Binding Protein 2 Regulates Hippo Signaling To Control Growth in Breast Epithelial Cells.

Li F, Bullough KZ, Vashisht AA, Wohlschlegel JA, Philpott CC
Molecular and cellular biology 2016 Aug 15;36(16):2121-31

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Iron Export through the Transporter Ferroportin 1 Is Modulated by the Iron Chaperone PCBP2.

Yanatori I, Richardson DR, Imada K, Kishi F
The Journal of biological chemistry 2016 Aug 12;291(33):17303-18

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hnRNP L and NF90 interact with hepatitis C virus 5'-terminal untranslated RNA and promote efficient replication.

Li Y, Masaki T, Shimakami T, Lemon SM
Journal of virology 2014 Jul;88(13):7199-209

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Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1 protects against antisense RNA and microRNAs.

Ho JJ, Robb GB, Tai SC, Turgeon PJ, Mawji IA, Man HS, Marsden PA
Molecular and cellular biology 2013 May;33(10):2029-46

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Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.

Fujimura K, Sasaki AT, Anderson P
Nucleic acids research 2012 Sep;40(16):8099-110

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Activation of the HIF prolyl hydroxylase by the iron chaperones PCBP1 and PCBP2.

Nandal A, Ruiz JC, Subramanian P, Ghimire-Rijal S, Sinnamon RA, Stemmler TL, Bruick RK, Philpott CC
Cell metabolism 2011 Nov 2;14(5):647-57

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Poly(C)-binding protein 2 interacts with sequences required for viral replication in the hepatitis C virus (HCV) 5' untranslated region and directs HCV RNA replication through circularizing the viral genome.

Wang L, Jeng KS, Lai MM
Journal of virology 2011 Aug;85(16):7954-64

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Identification of RNA helicase A as a new host factor in the replication cycle of foot-and-mouth disease virus.

Lawrence P, Rieder E
Journal of virology 2009 Nov;83(21):11356-66

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Selective localization of PCBP2 to cytoplasmic processing bodies.

Fujimura K, Katahira J, Kano F, Yoneda Y, Murata M
Biochimica et biophysica acta 2009 May;1793(5):878-87

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Rapid changes of mRNA-binding protein levels following glucose and 3-isobutyl-1-methylxanthine stimulation of insulinoma INS-1 cells.

Süss C, Czupalla C, Winter C, Pursche T, Knoch KP, Schroeder M, Hoflack B, Solimena M
Molecular & cellular proteomics : MCP 2009 Mar;8(3):393-408

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Identification of PCBP2, a facilitator of IRES-mediated translation, as a novel constituent of stress granules and processing bodies.

Fujimura K, Kano F, Murata M
RNA (New York, N.Y.) 2008 Mar;14(3):425-31

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A link between SIN1 (MAPKAP1) and poly(rC) binding protein 2 (PCBP2) in counteracting environmental stress.

Ghosh D, Srivastava GP, Xu D, Schulz LC, Roberts RM
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 19;105(33):11673-8

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Western blot analysis of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi or non-transfected control. Blot probed with H00005094-M07. GAPDH (36.1 kDa) used as loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody (M07), clone 5F12. Lane 1: PCBP2 transfected lysatE (38.7 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of PCBP2 expression in K-562 (Cat # L009V1).

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: PCBP2 Antibody (5F12) [H00005094-M07] - Detection limit for recombinant GST tagged PCBP2 is approximately 0.1ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of monoclonal antibody to PCBP2 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of monoclonal antibody to PCBP2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml