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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-379 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-379, RRID:AB_10000802
- Product name
- Rabbit Polyclonal CKII alpha prime polypeptide Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Unbalanced expression of CK2 kinase subunits is sufficient to drive epithelial-to-mesenchymal transition by Snail1 induction.
Casein kinase 2 reverses tail-independent inactivation of kinesin-1.
PKCδ-induced PU.1 phosphorylation promotes hematopoietic stem cell differentiation to dendritic cells.
Casein kinase 2 Is activated and essential for Wnt/beta-catenin signaling.
Deshiere A, Duchemin-Pelletier E, Spreux E, Ciais D, Combes F, Vandenbrouck Y, Couté Y, Mikaelian I, Giusiano S, Charpin C, Cochet C, Filhol O
Oncogene 2013 Mar 14;32(11):1373-83
Oncogene 2013 Mar 14;32(11):1373-83
Casein kinase 2 reverses tail-independent inactivation of kinesin-1.
Xu J, Reddy BJ, Anand P, Shu Z, Cermelli S, Mattson MK, Tripathy SK, Hoss MT, James NS, King SJ, Huang L, Bardwell L, Gross SP
Nature communications 2012 Mar 27;3:754
Nature communications 2012 Mar 27;3:754
PKCδ-induced PU.1 phosphorylation promotes hematopoietic stem cell differentiation to dendritic cells.
Hamdorf M, Berger A, Schüle S, Reinhardt J, Flory E
Stem cells (Dayton, Ohio) 2011 Feb;29(2):297-306
Stem cells (Dayton, Ohio) 2011 Feb;29(2):297-306
Casein kinase 2 Is activated and essential for Wnt/beta-catenin signaling.
Gao Y, Wang HY
The Journal of biological chemistry 2006 Jul 7;281(27):18394-400
The Journal of biological chemistry 2006 Jul 7;281(27):18394-400
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of Human CKII Alpha on HeLa WCL using NB100-379. IPed CKII alpha was detected using another goat anti-CKII alpha antibody. CKII alpha and XRCC1 were detected with NB100-379.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of Human and Mouse CKII alpha' by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of human CKII alpha' by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for IP at 6 ug per reaction. CKII alpha' was also immunoprecipitated by rabbit anti-CKII alpha' antibody NB100-378. For blotting immunoprecipitated CKII alpha', NB100-379 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
