PAB21289

antibody from Abnova Corporation

Targeting: INIP C9orf80, HSPC043, hSSBIP1, MISE, SOSS-C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21289

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21289, RRID:AB_10965938

Product name

C9orf80 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant C9orf80.

Antigen sequence

KEKRKLLMQNQSSTNHPGASIALSRPSLNKDFRDH
AEQQHIAAQQKAALQHAHAHSSGYFITQDSAFGNL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with C9orf80 polyclonal antibody (Cat # PAB21289) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with C9orf80 polyclonal antibody (Cat # PAB21289) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human adrenal gland with C9orf80 polyclonal antibody (Cat # PAB21289) shows strong nuclear positivity in cortical cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)