Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-28764 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FSHR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, Raji. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Two human menopausal gonadotrophin (hMG) preparations display different early signaling in vitro.
Casarini L, Riccetti L, Paradiso E, Benevelli R, Lazzaretti C, Sperduti S, Melli B, Tagliavini S, Varani M, Trenti T, Morini D, Falbo A, Villani MT, Jonas KC, Simoni M
Molecular human reproduction 2020 Dec 10;26(12):894-905
Molecular human reproduction 2020 Dec 10;26(12):894-905
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FSH Receptor using 30 µg of A) A431 (B) H1299 (C) HeLa and D) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a FSH Receptor polyclonal antibody (Product # PA5-28764) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FSHR Polyclonal Antibody (Product # PA5-28764). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FSHR Polyclonal Antibody (Product # PA5-28764) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FSHR Polyclonal Antibody (Product # PA5-28764). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FSHR Polyclonal Antibody (Product # PA5-28764) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FSHR Polyclonal Antibody detects FSH Receptor protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FSH Receptor protein stained by FSHR Polyclonal Antibody (Product # PA5-28764) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.