MAB8660

antibody from R&D Systems

Targeting: BST2 CD317, tetherin

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8660

Provider

R&D Systems

Product name

Mouse BST-2/Tetherin Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from cell culture supernatant. Detects mouse BST-2/Tetherin in flow cytometry.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

44E9R

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

BST-2/Tetherin in Mouse Splenocytes. BST-2/Tetherin was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse BST-2/Tetherin Monoclonal Antibody (Catalog # MAB8660) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of BST-2/Tetherin in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse BST-2/Tetherin Monoclonal Antibody (Catalog # MAB8660) or (B) Rat IgG_2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).