Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA500700 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500700, RRID:AB_11140959
- Product name
- GLUL mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)
- Antibody type
- Monoclonal
- Description
- GLUL mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GLUL
- Isotype
- IgG
- Antibody clone number
- OTI1F4
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references MYC regulates the unfolded protein response and glucose and glutamine uptake in endocrine resistant breast cancer.
Shajahan-Haq AN, Cook KL, Schwartz-Roberts JL, Eltayeb AE, Demas DM, Warri AM, Facey CO, Hilakivi-Clarke LA, Clarke R
Molecular cancer 2014 Oct 23;13:239
Molecular cancer 2014 Oct 23;13:239
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLUL (RC204161, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLUL.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-GLUL monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western blot analysis of GLUL protein level by using anti-GLUL antibody in LCC9 cells were treated with 10058-F4 (25 uM) or vehicle for 48 h or transfected with MYC or control siRNA for 48 h. Knockdown of MYC increased GLS/GAC levels and decreased GLUL levels.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-GLUL mouse monoclonal antibody (TA500700).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GLUL mouse monoclonal antibody (TA500700) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GLUL(RC204161).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GLUL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500700)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GLUL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500700)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GLUL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500700)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-GLUL antibody(TA500700),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC