AM1854a

antibody from Abgent

Targeting: RPS6KB2 KLS, P70-BETA, p70S6Kb, S6KB, S6Kbeta, S6Kβ, STK14B

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1854a

Provider

Abgent

Proper citation

Abgent Cat#AM1854a, RRID:AB_10816997

Product name

RPS6KB2 Antibody (Ascites)

Antibody type

Monoclonal

Antigen

Recombinant Protein

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

100 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of lysates from Jurkat, MCF-7, K562, A549, mouse NIH/3T3 cell line (from left to right), using RPS6KB2 Antibody (Cat. #AM1854a). AM1854a was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane."

Primary Ab dilution

1:2000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

RPS6KB2 Antibody (Cat. #AM1854a) western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the RPS6KB2 antibody detected the RPS6KB2 protein (arrow).(1:2000)

Primary Ab dilution

1:1000~2000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

RPS6KB2 Monoclonal( Ascites) (Cat. #AM1854a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6KB2 Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

RPS6KB2 Monoclonal( Ascites) (Cat. #AM1854a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50