AF2987
antibody from R&D Systems
Targeting: RPS6KB2
KLS, P70-BETA, p70S6Kb, S6KB, S6Kbeta, S6Kβ, STK14B
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF2987 - Provider product page
- Provider
- R&D Systems
- Product name
- Human p70 S6 Kinase beta Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human p70 S6 Kinase beta in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human p70 S6 Kinase is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9UBS0
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human p70 S6 Kinase beta by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human p70 S6 Kinase beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p70 S6 Kinase beta at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- p70 S6 Kinase beta in MCF-7 Human Cell Line. p70 S6 Kinase beta was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human p70 S6 Kinase beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2987) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.