LS-C815698
antibody from LSBio
Targeting: PRPF6
ANT-1, bB152O15.1, C20orf14, hPrp6, Prp6, RP60, SNRNP102, TOM, U5-102K
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- LS-C815698 - Provider product page
- Provider
- LSBio
- Product name
- PRPF6 / TOM Antibody (Preservative Free) LS-C815698
- Antibody type
- Polyclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-PRPF6 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 119 kDa. (We are unsure as to the identity of these extra bands.)
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- PRPF6 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane C:0.5 mg Hela Whole Cell Lysate. 4 uL anti-PRPF6 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-PRPF6 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 106 kDa. Observed band size: 106 kDa.