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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF7349 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse CBFB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse CBFB in Western blots and recombinant human CBFB in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant human RUNX-1, -2, and -3 is observed.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q13951- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse CBFB by Western Blot. Western blot shows lysates of M1 mouse myeloid leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CBFB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7349) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CBFB at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CBFB in HUVEC Human Cells. CBFB was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human/Mouse CBFB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7349) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
