Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX42912 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX42912, RRID:AB_11176664
- Product name
- 14-3-3 beta/zeta (phospho Ser185) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Rabbit, Sheep
- Host
- Sheep
- Storage
- Keep as concentrated solution. For short-term storage, store at 4¢X C (up to 10 days). For long-term storage, aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles. This product may contain precipitation. Recommend microcentrifugation before use.
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot with Sheep anti-14-3-3 (pSER185) antibody (GTX42912)Lane 1, 2μg recombinant 14-3-3 betaLane 2, 2μg recombinant 14-3-3 zeta Lane 3, sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185The recombinant 14-3-3s are expressed in E.coli and are not phosphorylated