GTX42912

antibody from GeneTex

Targeting: SFN YWHAS

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX42912

Provider

GeneTex

Proper citation

GeneTex Cat#GTX42912, RRID:AB_11176664

Product name

14-3-3 beta/zeta (phospho Ser185) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Bovine, Rabbit, Sheep

Host

Sheep

Storage

Keep as concentrated solution. For short-term storage, store at 4¢X C (up to 10 days). For long-term storage, aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles. This product may contain precipitation. Recommend microcentrifugation before use.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot with Sheep anti-14-3-3 (pSER185) antibody (GTX42912)Lane 1, 2μg recombinant 14-3-3 betaLane 2, 2μg recombinant 14-3-3 zeta Lane 3, sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185The recombinant 14-3-3s are expressed in E.coli and are not phosphorylated