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- References [1]
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- Western blot [2]
- Immunocytochemistry [3]
- Flow cytometry [1]
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- Product number
- NBP1-37095 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-37095, RRID:AB_2168172
- Product name
- Mouse Monoclonal Peroxiredoxin 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Redox Sensitivities of Global Cellular Cysteine Residues under Reductive and Oxidative Stress.
Araki K, Kusano H, Sasaki N, Tanaka R, Hatta T, Fukui K, Natsume T
Journal of proteome research 2016 Aug 5;15(8):2548-59
Journal of proteome research 2016 Aug 5;15(8):2548-59
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Peroxiredoxin 1 Antibody (1D8) [NBP1-37095] - Cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PRDX1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Peroxiredoxin 1 Antibody (1D8) [NBP1-37095] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PRDX1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: 293T cell lysate Lane 3.: MCF7 cell lysate Lane 4.: K562 cell lysate Lane 5.: A431 cell lysate Lane 6.: A549 cell lysate Lane 7.: Jurkat cell lysate
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Peroxiredoxin 1 Antibody (1D8) [NBP1-37095] - Analysis of PRDX1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Peroxiredoxin 1 Antibody (1D8) [NBP1-37095] - Analysis of PRDX1 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Peroxiredoxin 1 Antibody (1D8) [NBP1-37095] - Analysis of PRDX1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Peroxiredoxin 1 Antibody (1D8) [NBP1-37095] - Analysis of PRDX1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
