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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- 10915-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTCF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: HIRSHTGER PFQCSLCSYA SRDTYKLKRH MRTHSGEKPY ECYICHARFT QSGTMKMHIL QKHTENVAKF HCPHCDTVIA RKSDLGVHLR KQHSYIEQGK KCRYCDAVFH ERYALIQHQK SHKNEKRFKC DQCDYACRQE RHMIMHKRTH TGEKPYACSH CDKTFRQKQL LDMHFKRYHD PNFVPAAFVC SKCGKTFTRR NTMARHADNC AGPDGVEGEN GGETKKSKRG RKRKMRSKKE DSSDSENAEP DLDDNEDEEE PAVEIEPEPE PQPVTPAPPP AKKRRGRPPG RTNQPKQNQP TAIIQVEDQN TGAIENIIVE VKKEPDAEPA EGEEEEAQPA ATDAPNGDLT PEMILSMMDR (369-727 aa encoded by BC014267)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.18 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293T cells were subjected to SDS PAGE followed by western blot with 10915-1-AP (CTCF Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- U-937 cells were subjected to SDS PAGE followed by western blot with 10915-1-AP (CTCF Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 10915-1-AP (CTCF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4T1 cells were subjected to SDS PAGE followed by western blot with 10915-1-AP (CTCF Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 10915-1-AP) (CTCF antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma using 10915-1-AP (CTCF antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CTCF (IP:10915-1-AP, 5ug; Detection:10915-1-AP 1:300) with MCF-7 cells lysate 2560ug.
