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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Product number
- NBP1-50027 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-50027, RRID:AB_10012217
- Product name
- Rabbit Polyclonal SENP7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Speckle-type POZ protein suppresses hepatocellular carcinoma cell migration and invasion via ubiquitin-dependent proteolysis of SUMO1/sentrin specific peptidase 7.
Ji P, Liang S, Li P, Xie C, Li J, Zhang K, Zheng X, Feng M, Li Q, Jiao H, Chi X, Zhao W, Zhang S, Wang X
Biochemical and biophysical research communications 2018 Jul 7;502(1):30-42
Biochemical and biophysical research communications 2018 Jul 7;502(1):30-42
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: SENP7 Antibody [NBP1-50027] - Detection of Human SENP7 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SENP7 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SENP7 was also immunoprecipitated by rabbit anti-SENP7 antibody BL10697, which recognizes an upstream epitope. For blotting immunoprecipitated SENP7, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
