936101

antibody from BioLegend

Targeting: TFEB bHLHe35, TCFEB

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

936101

Provider

BioLegend

Product name

Purified anti-TFEB

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W18197C

Vial size

25 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Whole cell extracts (15 µg protein) from NTERA-2 and HeLa (reduced expression controls), Ramos, Raji, Daudi and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-TFEB antibody (clone W18197C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker. Cell lines were loaded in order of increasing predicted TFEB expression (source: Human Protein Atlas).

Conjugate

Unconjugated