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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00007942-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007942-M01, RRID:AB_425753
- Product name
- TFEB monoclonal antibody (M01), clone S1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant TFEB.
- Antigen sequence
MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQ
QQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVL
KVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFA
AHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAP
NSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYIN
PEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSS
SCPADLTQKRELTDAESRALAKERQKKDNHNLIER
RRRFNINDRIKELGMLIPKANDLDVRWNKGTILKA
SVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLR
IQELEMQARVHGLPTTSPSGMNMAELAQQVVKQEL
PSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPT
QPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPG
HGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMS
PEASKASSRRSSFSMEEGDVL- Isotype
- IgG
- Antibody clone number
- S1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Neuraminidase of Influenza A Virus Binds Lysosome-Associated Membrane Proteins Directly and Induces Lysosome Rupture.
Ju X, Yan Y, Liu Q, Li N, Sheng M, Zhang L, Li X, Liang Z, Huang F, Liu K, Zhao Y, Zhang Y, Zou Z, Du J, Zhong Y, Zhou H, Yang P, Lu H, Tian M, Li D, Zhang J, Jin N, Jiang C
Journal of virology 2015 Oct;89(20):10347-58
Journal of virology 2015 Oct;89(20):10347-58
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TFEB monoclonal antibody (M01), clone S1. Western Blot analysis of TFEB expression in rat brain.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TFEB is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to TFEB on HeLa cell. [antibody concentration 20 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
