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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
Reference
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- Product number
- GTX113492 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113492, RRID:AB_11163945
- Product name
- MAX antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE GTX113492 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX antibody (GTX113492) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MAX antibody immunoprecipitates MAX protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 £gg of preimmune rabbit IgG B. Immunoprecipitation of MAX protein by 2 £gg of MAX antibody (GTX113492) 15% SDS-PAGE The immunoprecipitated MAX protein was detected by MAX antibody (GTX113492) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
