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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00010165-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010165-M01, RRID:AB_670562
- Product name
- Mouse Monoclonal SLC25A13 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. solute carrier family 25, member 13 (citrin)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Diversion of aspartate in ASS1-deficient tumours fosters de novo pyrimidine synthesis.
Rabinovich S, Adler L, Yizhak K, Sarver A, Silberman A, Agron S, Stettner N, Sun Q, Brandis A, Helbling D, Korman S, Itzkovitz S, Dimmock D, Ulitsky I, Nagamani SC, Ruppin E, Erez A
Nature 2015 Nov 19;527(7578):379-383
Nature 2015 Nov 19;527(7578):379-383
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SLC25A13 Antibody (4F4) [H00010165-M01] - Analysis of SLC25A13 over-expressed 293 cell line, cotransfected with SLC25A13 Validated Chimera RNAi ( Cat # H00010165-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SLC25A13 monoclonal antibody (M01), clone 4F4 (Cat # H00010165-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SLC25A13 Antibody (4F4) [H00010165-M01] - Analysis of SLC25A13 expression in transfected 293T cell line by SLC25A13 monoclonal antibody (M01), clone 4F4.Lane 1: SLC25A13 transfected lysate(74.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SLC25A13 Antibody (4F4) [H00010165-M01] - SLC25A13 monoclonal antibody (M01), clone 4F4 Analysis of SLC25A13 expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SLC25A13 Antibody (4F4) [H00010165-M01] - Detection limit for recombinant GST tagged SLC25A13 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SLC25A13 Antibody (4F4) [H00010165-M01] - Analysis of monoclonal antibody to SLC25A13 on HepG2 cell. Antibody concentration 10 ug/ml.
