Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ASR-036-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 5HT4 Receptor (HTR4) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.85 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain lysate: - 1. Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (#ASR-036), (1:200). 2. Anti-5HT4 Receptor (HTR4) (extracellular) Antibody , preincubated with 5HT4 Receptor/HTR4 (extracellular) Blocking Peptide (#BLP-SR036).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Serotonin receptor 4 in rat PC12 cells - Cell surface detection of Serotonin receptor 4 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-5HT4 Receptor (HTR4) (extracellular) Antibody , (#ASR-036), (1:100), (red). B. Merge of A with a live view of the cell.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of Serotonin receptor 4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-5HT4 Receptor (HTR4) (extracellular) Antibody , (#ASR-036), (2.5μg) + goat- Anti-rabbit-FITC.