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- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF594 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-21 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-21 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse (rm) IL-2, rmIL-4, rmIL-15, and recombinant human IL-21 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9ES17- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Prostaglandin E2 inhibits Tr1 cell differentiation through suppression of c-Maf.
Intestinal microbiota link lymphopenia to murine autoimmunity via PD-1+CXCR5-/dim B-helper T cell induction.
Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Interleukin-21 is critically required in autoimmune and allogeneic responses to islet tissue in murine models.
STAT3-dependent IL-21 production from T helper cells regulates hematopoietic progenitor cell homeostasis.
IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).
Cytokine-secreting follicular T cells shape the antibody repertoire.
Tumor-specific Th17-polarized cells eradicate large established melanoma.
IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner.
Expression and one-step purification of bovine interleukin-21 (IL-21) in silkworms using a hybrid baculovirus expression system.
IL-21 induces the functional maturation of murine NK cells.
IL-21 induces the functional maturation of murine NK cells.
IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
Hooper KM, Kong W, Ganea D
PloS one 2017;12(6):e0179184
PloS one 2017;12(6):e0179184
Intestinal microbiota link lymphopenia to murine autoimmunity via PD-1+CXCR5-/dim B-helper T cell induction.
Eri T, Kawahata K, Kanzaki T, Imamura M, Michishita K, Akahira L, Bannai E, Yoshikawa N, Kimura Y, Satoh T, Uematsu S, Tanaka H, Yamamoto K
Scientific reports 2017 Apr 26;7:46037
Scientific reports 2017 Apr 26;7:46037
Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
Interleukin-21 is critically required in autoimmune and allogeneic responses to islet tissue in murine models.
McGuire HM, Walters S, Vogelzang A, Lee CM, Webster KE, Sprent J, Christ D, Grey S, King C
Diabetes 2011 Mar;60(3):867-75
Diabetes 2011 Mar;60(3):867-75
STAT3-dependent IL-21 production from T helper cells regulates hematopoietic progenitor cell homeostasis.
Kaplan MH, Glosson NL, Stritesky GL, Yeh N, Kinzfogl J, Rohrabaugh SL, Goswami R, Pham D, Levy DE, Brutkiewicz RR, Blum JS, Cooper S, Hangoc G, Broxmeyer HE
Blood 2011 Jun 9;117(23):6198-201
Blood 2011 Jun 9;117(23):6198-201
IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).
Rynda A, Maddaloni M, Ochoa-Repáraz J, Callis G, Pascual DW
PloS one 2010 Jan 14;5(1):e8720
PloS one 2010 Jan 14;5(1):e8720
Cytokine-secreting follicular T cells shape the antibody repertoire.
Reinhardt RL, Liang HE, Locksley RM
Nature immunology 2009 Apr;10(4):385-93
Nature immunology 2009 Apr;10(4):385-93
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
Blood 2008 Jul 15;112(2):362-73
Blood 2008 Jul 15;112(2):362-73
IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner.
Wei L, Laurence A, Elias KM, O'Shea JJ
The Journal of biological chemistry 2007 Nov 30;282(48):34605-10
The Journal of biological chemistry 2007 Nov 30;282(48):34605-10
Expression and one-step purification of bovine interleukin-21 (IL-21) in silkworms using a hybrid baculovirus expression system.
Muneta Y, Nagaya H, Minagawa Y, Enomoto C, Matsumoto S, Mori Y
Biotechnology letters 2004 Sep;26(18):1453-8
Biotechnology letters 2004 Sep;26(18):1453-8
IL-21 induces the functional maturation of murine NK cells.
Brady J, Hayakawa Y, Smyth MJ, Nutt SL
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 15;172(4):2048-58
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 15;172(4):2048-58
IL-21 induces the functional maturation of murine NK cells.
Brady J, Hayakawa Y, Smyth MJ, Nutt SL
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 15;172(4):2048-58
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 15;172(4):2048-58
IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1540-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1540-7
IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1540-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1540-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-21 in Mouse Splenocytes. IL-21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-21 and Neutralization by Mouse IL-21 Antibody. Recombinant Mouse IL-21 (Catalog # 594-ML) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-21 (250 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594). The ND50 is typically 5-15 µg/mL.
