Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-29929 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal pIgR Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 0.6 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: pIgR Antibody [NBP2-29929] - Western blot analysis of PIGR Antibody (C-term) (NBP2-29929) in HepG2(lane 1),K562(lane 2),HL-60(lane 3) cell line lysates (35ug/lane).PIGR (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: pIgR Antibody [NBP2-29929] - Confocal immunofluorescent analysis of PIGR Antibody (C-term) (NBP2-29929) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: pIgR Antibody [NBP2-29929] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: pIgR Antibody [NBP2-29929] - Formalin-fixed and paraffin-embedded human hepatocarcinoma with PIGR Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: pIgR Antibody [NBP2-29929] - Analysis of HepG2 cells using PIGR Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.