H00008681-M01

antibody from Abnova Corporation

Targeting: JMJD7-PLA2G4B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008681-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008681-M01, RRID:AB_733236

Product name

PLA2G4B monoclonal antibody (M01), clone 1G5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PLA2G4B.

Antigen sequence

MAEAALEAVRSELREFPAAARELCVPLAVPYLDKP
PTPLHFYRDWVCPNRPCIIRNALQHWPALQKWSLP
YFRATVGSTEVSVAVTPDGYADAVRGDRFMMPAER
RLPLSFVLDVLEGRAQHPGVLYVQKQCSNLPSELP
QLLPDLESHVPWASEALGKMPDAVNFWLGEAAAVT
SLHKDHYENLYCVVSGEKHFLFHPPSDRPFIPYEL
YTPATYQLTEEGTFKVVDEEAMEKVPWIPLDPLAP
DLARYPSYSQAQALRCTVRAGEMLYLPALWFHHVQ
QSQGCIAVNFWYDMEYDLKYSYFQLLDSLTKASGL
D


Isotype

IgG

Antibody clone number

1G5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PLA2G4B expression in transfected 293T cell line by PLA2G4B monoclonal antibody (M01), clone 1G5.Lane 1: PLA2G4B transfected lysate(114.121 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PLA2G4B is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol