H00011155-M06-100ug

antibody from Novus Biologicals

Targeting: LDB3 CMD1C, KIAA0613, PDLIM6, ZASP

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00011155-M06-100ug

Provider

Novus Biologicals

Product name

Mouse Monoclonal LDB3 Antibody

Antibody type

Monoclonal

Description

IgG purified. LDB3 (3C8)

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LDB3 Antibody (3C8) [H00011155-M06] - Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi or non-transfected control. Blot probed with H00011155-M06. GAPDH (36.1 kDa) used as loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LDB3 Antibody (3C8) [H00011155-M06] - Analysis of LDB3 expression in transfected 293T cell line by LDB3 monoclonal antibody (M06), clone 3C8. Lane 1: LDB3 transfected lysatE (31 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LDB3 Antibody (3C8) [H00011155-M06] - Analysis of LDB3 expression in A-431 (Cat # L015V1).

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: LDB3 Antibody (3C8) [H00011155-M06] - Detection limit for recombinant GST tagged LDB3 is approximately 0.03ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: LDB3 Antibody (3C8) [H00011155-M06] - Analysis of monoclonal antibody to LDB3 on A-431 cell. Antibody concentration 10 ug/ml