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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-52249 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRPS22 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TKKPTFMDEE VQSILTKMTG LNLQKTFKPA IQELKPPTYK LMTQAQLEEA TRQAVEAAKV RLKMPPVLEE RVPINDVLAE DKILEGTETT KYVFTDISYS IPHRERFIVV REPSGTLRKA
- Concentration
- 0.2 mg/mL
Submitted references The mitochondrial type IB topoisomerase drives mitochondrial translation and carcinogenesis.
Baechler SA, Factor VM, Dalla Rosa I, Ravji A, Becker D, Khiati S, Miller Jenkins LM, Lang M, Sourbier C, Michaels SA, Neckers LM, Zhang HL, Spinazzola A, Huang SN, Marquardt JU, Pommier Y
Nature communications 2019 Jan 8;10(1):83
Nature communications 2019 Jan 8;10(1):83
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRPS22 in Lane 1: Marker (kDa) 229, 112, 84, 48, 32, 27, 17; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a MRPS22 Polyclonal Antibody (Product # PA5-52249).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRPS22 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a MRPS22 Polyclonal Antibody (Product # PA5-52249).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MRPS22 in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using a MRPS22 Polyclonal Antibody (Product # PA5-52249).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MRPS22 in human kidney using MRPS22 Polyclonal Antibody (Product # PA5-52249).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MRPS22 in human cerebral cortex, colon, kidney and testis using MRPS22 Polyclonal Antibody (Product # PA5-52249) (A) shows similar protein distribution across tissues to an independent MRPS22 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MRPS22 in human cerebral cortex using MRPS22 Polyclonal Antibody (Product # PA5-52249).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MRPS22 in human colon using MRPS22 Polyclonal Antibody (Product # PA5-52249).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MRPS22 in human testis using MRPS22 Polyclonal Antibody (Product # PA5-52249).
