12781-1-AP

antibody from Invitrogen Antibodies

Targeting: CBLB Cbl-b, RNF56

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12781-1-AP

Provider

Invitrogen Antibodies

Product name

CBLB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MANSMNGRN PGGRGGNPRK GRILGIIDAI QDAVGPPKQA AADRRTVEKT WKLMDKVVRL CQNPKLQLKN SPPYILDILP DTYQHLRLIL SKYDDNQKLA QLSENEYFKI YIDSLMKKSK RAIRLFKEGK ERMYEEQSQD RRNLTKLSLI FSHMLAEIKA IFPNGQFQGD NFRITKADAA EFWRKFFGDK TIVPWKVFRQ CLHEVHQISS GLEAMALKST IDLTCNDYIS VFEFDIFTRL FQPWGSILRN WNFLAVTHPG YMAFLTYDEV KARLQKYSTK PGSYIFRLSC TRLGQWAIGY VTGDGNILQT IPHNKPLFQA LIDGSREGFY LYPDGRSYNP DLTGLCEPTP HDH (1-352 aa encoded by BC032851)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CBLB (IP:12781-1-AP, 4ug; Detection:12781-1-AP 1:300) with HEK-293 cells lysate 3680ug.