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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB0486 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0486, RRID:AB_982445
- Product name
- ERBB4 (phospho Y1162) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERBB4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cell surface expression of epidermal growth factor receptor and Her-2 with nuclear expression of Her-4 in primary osteosarcoma.
Changes in the expression and cytological localization of betacellulin and its receptors (ErbB-1 and ErbB-4) in the trophoblasts in human placenta over the course of pregnancy.
Ectodomain cleavage of ErbB-4: characterization of the cleavage site and m80 fragment.
Hughes DP, Thomas DG, Giordano TJ, Baker LH, McDonagh KT
Cancer research 2004 Mar 15;64(6):2047-53
Cancer research 2004 Mar 15;64(6):2047-53
Changes in the expression and cytological localization of betacellulin and its receptors (ErbB-1 and ErbB-4) in the trophoblasts in human placenta over the course of pregnancy.
Tanimura K, Nakago S, Murakoshi H, Takekida S, Moriyama T, Matsuo H, Hashimoto K, Maruo T
European journal of endocrinology / European Federation of Endocrine Societies 2004 Jul;151(1):93-101
European journal of endocrinology / European Federation of Endocrine Societies 2004 Jul;151(1):93-101
Ectodomain cleavage of ErbB-4: characterization of the cleavage site and m80 fragment.
Cheng QC, Tikhomirov O, Zhou W, Carpenter G
The Journal of biological chemistry 2003 Oct 3;278(40):38421-7
The Journal of biological chemistry 2003 Oct 3;278(40):38421-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ERBB4 (phospho Y1162) polyclonal antibody (Cat # PAB0486) was used to detect Phospho-ERBB4-Y1162 in FG pancreatic carcinoma cell lysate. FG pancreatic carcinoma cells treated with or without EGF (50 ng/ml) for 15 min ERBB4 (phospho Y1162) polyclonal antibody (Cat # PAB0486) was used with 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ERBB4 (phospho Y1162) polyclonal antibody (Cat # PAB0486) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
