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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX129505 - Provider product page
- Provider
- GeneTex
- Product name
- TRRAP antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- TRRAP antibody detects TRRAP protein by western blot analysis.A. 30 £gg Jurkat whole cell lysate/extract B. 30 £gg Raji whole cell lysate/extract C. 30 £gg NCI-H929 whole cell lysate/extract5 % SDS-PAGETRRAP antibody (GTX129505) dilution: 1:500
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- GeneTex (provider)
- Main image
- Experimental details
- TRRAP antibody detects TRRAP protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract D. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGETRRAP antibody (GTX129505) dilution: 1:500
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- TRRAP antibody detects TRRAP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRRAP protein stained by TRRAP antibody (GTX129505) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- TRRAP antibody immunoprecipitates TRRAP protein in IP experiments.IP samples: 293T whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of TRRAP protein by 4 £gg TRRAP antibody (GTX129505)C : Immunoprecipitation of TRRAP by 4 £gg of TRRAP antibody (GTX129507) 5 % SDS-PAGEThe immunoprecipitated TRRAP protein was detected by TRRAP antibody (GTX129505) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 £gg of either control rabbit IgG or anti-TRRAP antibody. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.
