PA5-85580

antibody from Invitrogen Antibodies

Targeting: TRRAP PAF400, TR-AP, Tra1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85580

Provider

Invitrogen Antibodies

Product name

TRRAP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TRRAP in A) Jurkat whole cell lysate, B) Raji whole cell lysate, C) NCI-H929 whole cell lysate using TRRAP polyclonal antibody (Product # PA5-85580) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TRRAP Polyclonal Antibody detects TRRAP protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.5 % SDS-PAGE. TRRAP Polyclonal Antibody (Product # PA5-85580) dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TRRAP Polyclonal Antibody detects TRRAP protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract. C. 30 µg NCI-H929 whole cell lysate/extract.5 % SDS-PAGE. TRRAP Polyclonal Antibody (Product # PA5-85580) dilution: 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TRRAP Polyclonal Antibody detects TRRAP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRRAP protein stained by TRRAP Polyclonal Antibody (Product # PA5-85580) diluted at 1:1,000. Blue: Hoechst 33342 staining.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP (chromatin immunoprecipitation) analysis of TRRAP in HeLa chromatin extract with TRRAP polyclonal antibody (Product # PA5-85580) using 5 µg of sample.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or TRRAP Polyclonal Antibody (Product # PA5-85580). The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of TRRAP in A) control of preimmune rabbit IgG, B) immunoprecipitation of TRRAP protein, C) immunoprecipitation of TRRAP protein with TRRAP polyclonal antibody (Product # PA5-85580) using 4 µg of sample at a dilution of 1:500. Sample was then incubated with anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.