Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- PB9876 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Nucleobindin 2/NUCB2 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for NESFATIN 1/Nucb2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Mouse. NESFATIN 1/Nucb2 information: Molecular Weight: 50304 MW; Subcellular Localization: Cytoplasm . Perikaryon . Endoplasmic reticulum . Golgi apparatus . Nucleus envelope . Membrane ; Peripheral membrane protein . Secreted . In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted; Tissue Specificity: Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Nucleobindin 2 was detected in paraffin-embedded sections of mouse gaster tissues using rabbit anti- Nucleobindin 2 Antigen Affinity purified polyclonal antibody (Catalog # PB9876) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
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