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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-34831 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASAH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, mouse heart, mouse muscle. Predicted reactivity: Mouse (81%), Rat (84%), Chimpanzee (100%), Bovine (88%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.03 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ASAH1 using 30 µg of NT2D1 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ASAH1 polyclonal antibody (Product # PA5-34831) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ASAH1 Polyclonal Antibody (Product # PA5-34831). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASAH1 Polyclonal Antibody (Product # PA5-34831) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ASAH1 Polyclonal Antibody detects ASAH1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ASAH1 stained by ASAH1 Polyclonal Antibody (Product # PA5-34831) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using ASAH1 (Product # PA5-34831) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
