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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00000427-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000427-M02, RRID:AB_2289828
- Product name
- Mouse Monoclonal ASAH1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ASAH1 - N-acylsphingosine amidohydrolase (acid ceramidase) 1 (1A7)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASAH1 Antibody (1A7) [H00000427-M02] - Analysis of ASAH1 expression in transfected 293T cell line by ASAH1 monoclonal antibody (M02), clone 1A7.Lane 1: ASAH1 transfected lysate(45 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ASAH1 Antibody (1A7) [H00000427-M02] - Detection limit for recombinant GST tagged ASAH1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASAH1 Antibody (1A7) [H00000427-M02] - Analysis of monoclonal antibody to ASAH1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
