Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
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- Product number
- NBP1-89296 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89296, RRID:AB_11025126
- Product name
- Rabbit Polyclonal ASAH1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ASAH1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A Guanidine-Based Synthetic Compound Suppresses Angiogenesis via Inhibition of Acid Ceramidase.
Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells.
Acid ceramidase (ASAH1) represses steroidogenic factor 1-dependent gene transcription in H295R human adrenocortical cells by binding to the receptor.
Acid ceramidase (ASAH1) is a global regulator of steroidogenic capacity and adrenocortical gene expression.
Genistein stimulates MCF-7 breast cancer cell growth by inducing acid ceramidase (ASAH1) gene expression.
The cAMP-responsive element binding protein (CREB) regulates the expression of acid ceramidase (ASAH1) in H295R human adrenocortical cells.
Cho SM, Lee HK, Liu Q, Wang MW, Kwon HJ
ACS chemical biology 2019 Jan 18;14(1):11-19
ACS chemical biology 2019 Jan 18;14(1):11-19
Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells.
Cai K, Lucki NC, Sewer MB
Biochimica et biophysica acta 2014 Apr 4;1841(4):552-62
Biochimica et biophysica acta 2014 Apr 4;1841(4):552-62
Acid ceramidase (ASAH1) represses steroidogenic factor 1-dependent gene transcription in H295R human adrenocortical cells by binding to the receptor.
Lucki NC, Li D, Bandyopadhyay S, Wang E, Merrill AH, Sewer MB
Molecular and cellular biology 2012 Nov;32(21):4419-31
Molecular and cellular biology 2012 Nov;32(21):4419-31
Acid ceramidase (ASAH1) is a global regulator of steroidogenic capacity and adrenocortical gene expression.
Lucki NC, Bandyopadhyay S, Wang E, Merrill AH, Sewer MB
Molecular endocrinology (Baltimore, Md.) 2012 Feb;26(2):228-43
Molecular endocrinology (Baltimore, Md.) 2012 Feb;26(2):228-43
Genistein stimulates MCF-7 breast cancer cell growth by inducing acid ceramidase (ASAH1) gene expression.
Lucki NC, Sewer MB
The Journal of biological chemistry 2011 Jun 3;286(22):19399-409
The Journal of biological chemistry 2011 Jun 3;286(22):19399-409
The cAMP-responsive element binding protein (CREB) regulates the expression of acid ceramidase (ASAH1) in H295R human adrenocortical cells.
Lucki N, Sewer MB
Biochimica et biophysica acta 2009 Aug;1791(8):706-13
Biochimica et biophysica acta 2009 Aug;1791(8):706-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASAH1 Antibody [NBP1-89296] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-ASAH1 antibody. Corresponding ASAH1 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human kidney shows strong granular cytoplasmic positivity in a subset of renal tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.