Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [10]
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Validation data
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- Product number
- NBP1-80754 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-80754, RRID:AB_11031453
- Product name
- Rabbit Polyclonal SHMT2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SHMT2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance.
Kim D, Fiske BP, Birsoy K, Freinkman E, Kami K, Possemato RL, Chudnovsky Y, Pacold ME, Chen WW, Cantor JR, Shelton LM, Gui DY, Kwon M, Ramkissoon SH, Ligon KL, Kang SW, Snuderl M, Vander Heiden MG, Sabatini DM
Nature 2015 Apr 16;520(7547):363-7
Nature 2015 Apr 16;520(7547):363-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHMT2 Antibody [NBP1-80754] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHMT2 Antibody [NBP1-80754] - Analysis in human cell line RH-30.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHMT2 Antibody [NBP1-80754] - Analysis using Anti-SHMT2 antibody NBP1-80754 (A) shows similar pattern to independent antibody NBP1-80755 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human lymph node shows moderate granular cytoplasmic positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human stomach shows moderate granular cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining in human liver and skeletal muscle tissues . Corresponding SHMT2 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human skin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human colon, kidney, liver and skin using Anti-SHMT2 antibody NBP1-80754 (A) shows similar protein distribution across tissues to independent antibody NBP1-80755 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human liver using Anti-SHMT2 antibody NBP1-80754.