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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-54454 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHLP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EISSGEGFLD MIDKEQKSIV IMVHIYEDGI PGTEAMNGCM ICLAAEYPAV KFCKVK
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHLP in control (vector only transfected HEK293T lysate) and PDCL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a PHLP Polyclonal Antibody (Product # PA5-54454).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PHLP in human cell line U-251 MG shows positivity in cytoplasm. Samples were probed using a PHLP Polyclonal Antibody (Product # PA5-54454).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PHLP in human skeletal muscle shows distinct cytoplasmic positivity in myocytes. Samples were probed using a PHLP Polyclonal Antibody (Product # PA5-54454).
