AP8691b

antibody from Abgent

Targeting: NIP7 CGI-37, FLJ10296, HSPC031, KD93

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP8691b

Provider

Abgent

Proper citation

Abgent Cat#AP8691b, RRID:AB_10551852

Product name

NIP7 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.42 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of NIP7 Antibody (C-term) (Cat. #AP8691b) in NIH-3T3 cell line lysates (35ug/lane). NIP7 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of NIP7 (arrow) using rabbit polyclonal NIP7 Antibody (C-term) (Cat. #AP8691b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NIP7 gene.

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

NIP7 Antibody (C-term) (Cat. #AP8691b) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50